Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

Cryopreservation by droplet vitrification was applied to hazelnut (Corylus avellana L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from in vitro growing shoots, immersed ice cooled PVS2 or PVS3 for 60 90 min, then transferred a solution, placed on strip aluminium foil, and plunged into liquid nitrogen (LN). Additionally, effect recovery mother plant after cryopreservation evaluated, following cold pre-treatment at 4 °C 3 months. The highest regrowth percentage (56.7%) obtained applying while application same amount time reduced 41.5%. Increasing exposure solutions min 43.3% when applied, 35.6% if used. did not significantly improve overall regrowth. process decline rooting ability recovered shoots.